Humanin

Quick Facts

• Full Name: Humanin (HN)
• Aliases: HN, Humanin-G (HNG — potent glycine-14 analog)
• Category: Longevity / Mitochondrial Health / Neuroprotection
• Sequence: 21 amino acids (mitochondrial-derived peptide, encoded in 16S rRNA region)
• Molecular Weight: ~2.8 kDa
• Administration: Subcutaneous / Intranasal (research)
• Typical Dose: 2–4 mg/week SubQ (human extrapolation; doses vary widely)
• Half-Life: Short; multiple dosing strategies explored
• Status: Research Only
• WADA Status: Not currently listed

What It Does

Humanin is a small peptide encoded within the mitochondrial genome (16S rRNA region), making it one of a class called mitochondrial-derived peptides (MDPs). It is cytoprotective across multiple tissues — brain, heart, vasculature, and metabolic organs. Humanin levels decline with age and are inversely correlated with Alzheimer's disease risk and metabolic dysfunction. It is considered a key longevity signaling molecule and a bridge between mitochondrial health and systemic aging.

Mechanism of Action

1. Anti-apoptotic signaling: Activates JAK2/STAT3, PI3K/Akt, and MAPK pathways → inhibits Bax-mediated mitochondrial apoptosis
2. Neuronal protection: Blocks amyloid-beta (Aβ) toxicity in neurons; reduces tau pathology markers
3. Insulin sensitization: Activates FPRL1/FPR2 and gp130 receptors → improves insulin sensitivity and glucose metabolism
4. Mitochondrial function: Supports mitochondrial membrane potential and reduces ROS production
5. IGF-1 modulation: Interacts with IGFBP-3, modulating IGF-1 bioavailability and longevity signaling
6. Systemic cytoprotection: Cardioprotective, hepatoprotective, and vasculoprotective effects reported

Ageless Peps Products

• APWS-Humanin-Vial — Humanin 10mg Vial, $60, wholesale only

Dosing Protocols

Research-Informed Protocol

• Dose: 2–4 mg/week SubQ (divided doses)
• Frequency: 2–3x per week
• Duration: Ongoing cycles with periodic assessment
• Note: Human dosing extrapolated from rodent studies; clinical protocols not yet established
• Source: The Peptides Bible, longevity research literature

Stacks Well With

• TB-500 – systemic cytoprotection and tissue repair synergy
• Melanotan II – complementary mitochondrial and metabolic signaling
• MOTS-C – fellow mitochondrial-derived peptide; complementary longevity signaling
• Epitalon – anti-aging and telomere support synergy
• SS-31 – mitochondrial membrane protector; powerful MDP stack

Use Cases

• Longevity and healthy aging (MDP decline reversal)
• Neuroprotection (Alzheimer's risk reduction, neuronal survival)
• Metabolic Health (insulin sensitization, glucose metabolism)
• Cardiovascular Protection
• Mitochondrial Health optimization
• Cognitive Function maintenance

Contraindications & Safety

• Active malignancy — anti-apoptotic signaling; theoretical concern
• Limited human data — extrapolated from rodent studies; use cautiously
• Drug interactions — JAK/STAT pathway involvement; caution with immunosuppressants
• Common side effects: Not well characterized in humans; generally well-tolerated in animal models
• Long-term safety profile in humans not established

Storage

• Lyophilized: -20°C long-term; 2–8°C up to 3 months
• Reconstituted: 2–8°C for 1–2 weeks; protect from light
• Solvent: Sterile/bacteriostatic water

PubMed Research

Research links will be added after PubMed search

Sources

• The Peptides Bible – Mitochondrial peptides section
• Peptide Protocols Master Bible – Longevity protocols
• Longevity research literature (Cohen et al., 2013; Bhanu et al.)

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